NL5
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Get fast and high resolution images with NL5
Low phototoxicity, the solution for life cell imaging
The technology of the NL5 was born in the ’80s. New developments such as advances in CMOs cameras have made it possible to take full advantage of the optics of this system, making it fast and sensitive. The NL5 can capture images with speeds that reach up to 25 fps (full frame), much faster than regular point-scanning confocal systems.
The NL5 is easily added onto your existing widefield microscope and is housed in a very compact package. The increased speed is due to the usage of a slit pinhole which allows the scanning of the sample line by line, in contrast to the point by point-scanning of conventional confocal systems. This allows the NL5 to capture images even faster by scanning a full line instead of a single point.
Due to its fast scanning abilities, your valuable samples are exposed to light only for a brief moment. This prevents damaging your samples by phototoxicity, keeping your samples happy under the microscope for a very long time.
Combine the NL5 system with the latest CMOS camera and you will be able to reduce the laser power even further. This is thanks to the high quantum efficiency of modern CMOS cameras. With this combination, you can capture high-resolution images with a high signal-to-noise ratio. All of this, without any sign of photobleaching and phototoxicity. Do you need advice on what CMOS camera to use alongside the NL5? Feel free to contact us!
The perfect tool for fast and deep 3D live cell imaging
Due to it fast scanning capabilities
The NL5 can reach up to 240 nm lateral resolution which can be further improved by deconvolution to 170 nm. Due to the single slit pinhole of NL5, you get high contrast images even when going deeper into the sample, which makes this confocal solution ideal for deep 3D imaging.
Due to its high temporal resolution, with NL5 you can quickly capture multicolour 3D images of samples in multi-wellplates to select the most promising ones. NL5 system is a cost-effective solution that you can pair to any research microscope via a C-mount adapter.
Product features
Scans your sample with speeds up to 25 FPS
With the NL5 system there is no pinhole crosstalk, greatly increasing contrast and image quality
Due to the high sensitivity detector, NL5 requires low laser power which leads to low phototoxicity and bleaching
The NL5 is easily attachable to any research microscope via the c-mount interface
- Detector Camera
- Resolution 170 nm (after deconvolution)
- Sensitivity up to 95% QE
- FOV 330×330 μm (40x, magnification)
- Optimized for Low 10x-40x magnification and for high 40x-100x magnification
- Scanner Digital (closed-loop)
- Speed 25 fps full frame
- Wavelength VIS
- Software Micromanager, and 3rd party softwares such as Volocity, NIS Elements
- Integration API
- PSF for deconvolution with Microvolution
- Bypass mode - Motorized
Satisfied customers
That's why we do it!
Need more information about the NL5?
Don't hesitate to contact us
Wish to know more about the NL5? Wish to have a demonstration? Feel free to contact us today!
Request more info - Equipment
Product downloads
View the accompanying brochures
- NL5 Application Note Download
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